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Infertility or sub fertility affects 8–12% of couples worldwide of them 40–50% is due to “Male factor”, either solely (20%) or in combination with the female factor (30–40%) and another 30-40% are with no male-infertility-associated factor or idiopathic male infertility.

The primary objective of semen analysis is to assess the suitability of semen sample and to comment on total sperm concentration (TSC) and progressive motile (PR) active spermatozoa which predicts the proability of fertilization. Before starting an infertility treatment cycle (IUI/IVF) at least one diagnostic semen analysis should be performed according to the protocols described in the World Health Organization (WHO) manual, 2010. In addition, a test sperm preparation may be advisable in order to propose the most adequate insemination technique (IUI/IVF).

Spermatozoa may need to be separated from seminal plasma for variety of purposes, such as diagnostics tests of function and therapeutic recovery of insemination and assisted reproductive technologies (ART). The separation of human spermatozoa from seminal plasma to yield a final preparation containing high percentage of morphologically normal and motile cells, free from debris, non-germ cells and dead spermatozoa. Diluting semen with culture media and centrifuging is still used for preparing normozoospermic specimens for IUI.

Sperm preparation techniques eliminate seminal plasma, debris and contaminants, concentrate progressively motile sperm and select morphologically abnormal sperm.


Husband Semen Analysis (as per 2010 WHO standards)

Semen Sample Preparation (IUI)

Sperm Function Test (SFT)

Sperm Chromatin DNA fragmentation Index (DFI) Test

Y chromosome Microdeletion in Azoospermia & Oligozoospermia